Where are supersedure cells typically found in a hive?

Supersedure cells are specifically associated with the process of replacing an existing queen bee. They are typically found hanging off the middle of the frame. This location is important because it allows the new queen to develop and emerge in a part of the hive that is well-protected yet accessible for the workers to tend to the developing larvae.

Finding supersedure cells in this position indicates that the colony is actively choosing to replace its queen, signaling to the beekeeper a vital reproductive and management issue within the hive. Other locations, such as corners or the top of the hive, do not provide the same level of protection and support for the queen cells as the middle section inside the frames does. This positioning within the frames also aligns with the natural behavior of bees, as they tend to optimize their available space for significant colony activities such as queen rearing.